Untargeted Plasma Metabolite Profiling Reveals the Broad Systemic Consequences of Xanthine Oxidoreductase Inactivation in Mice

Untargeted Plasma Metabolite Profiling Reveals the Broad Systemic Consequences of Xanthine Oxidoreductase Inactivation in Mice

A major challenge in systems biology is integration of molecular findings for individual enzyme activities into a cohesive high-level understanding of cellular metabolism and physiology/pathophysiology. However, meaningful prediction for how a perturbed enzyme activity will globally impact metabolism in a cell, tissue or intact organisms is precluded by multiple unknowns, including in vivo enzy...

Metabolite profiling in blood plasma.

Metabolite profiling has been established as a multiparallel strategy for relative quantification of a mixture of compounds or compound classes using chromatography and universal detection technologies (gas chromatography-mass spectrometry [GC-MS], liquid chromatography-MS). Despite its origins dating back to the late 1960s, it was only in the 1980s that its use was acknowledged to diagnose met...

Regulation of the Human Xanthine Oxidoreductase Gene

The thermodynamics of xanthine oxidoreductase catalysis.

Xanthine oxidoreductase is a complex enzyme found in a wide range of organisms. Recent interest in this enzyme stems from its ability to produce reactive oxygen species under a range of conditions. It is found as a homodimer, each unit containing a molybdopterin cofactor, two iron sulfur centers, and FAD. The enzyme can exist in two forms that differ primarily in their oxidizing substrate speci...

Intestinal Microbial and Metabolite Profiling of Mice

1 CNC – Center for Neuroscience and Cell Biology, University of Coimbra, Portugal; 7 [email protected] 8 2 Institute of Microbiology, Faculty of Medicine, University of Coimbra, Portugal 9 3 CEDOC, NOVA Medical School, Universidade NOVA de Lisboa, Rua Camara Pestana, no6, 6A, edifício II, 10 piso 3, 1150-082 Lisbon, Portugal 11 4 CICECO-Department of Chemistry, University of Aveiro,...